To avoid having to pipette very small quantities (1-10 l) to make a dilution of a solution, serial dilutions are often used. Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators. The density of cells is lowered in each step of serial dilution, making it easier to calculate the concentration of cells in the initial solution by computing the total dilution over the series. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results. In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. In biology and medicine Serial dilution and plating of bacteria Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M. ![]() Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. They are identifiable with a special user flair.Ī community since MaAsking a question? Describe if you are using Excel (include version and operating system!), Google Sheets, or another spreadsheet application.Stepwise dilution of a substance in solution Logarithmic dilutionĪ serial dilution is the stepwise dilution of a substance in solution. ![]() Occasionally Microsoft developers will post or comment. Dilution factor amount of specimen transferred divided by the total volume after transferamount of specimen transferred + amount of diluent already in tube. I need to dilute the above solutions with water. The tool below can be used to create a protocol for preparing a serial dilution from a stock solution. For instance, creating a two-fold dilution with a starting concentration of 10 M yields the following concentrations: 10 M, 5 M, 2.5 M, 1.25 M, etc. I am starting with 15 (100lbs15lbs polymer+85lbs water), 12, and 10 solutions. A serial dilution is a sequence of dilutions created using the same dilution factor. I need to be able to enter a goal weight in to a cell (example: 100 (lbs)) of a (2) solution. ![]() Recent ClippyPoint Milestones !Ĭongratulations and thank you to these contributors Date I am attempting to create a spreadsheet to provide dilution measurements (by weight) of a polymer solution. Include a screenshot, use the tableit website, or use the ExcelToReddit converter (courtesy of u/tirlibibi17) to present your data. NOTE: For VBA, you can select code in your VBA window, press Tab, then copy and paste that into your post or comment. To keep Reddit from mangling your formulas and other code, display it using inline-code or put it in a code-block This will award the user a ClippyPoint and change the post's flair to solved. OPs can (and should) reply to any solutions with: Solution Verified
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